S. pneumoniae Topoisomerase IV

Topo IV (from Streptococcus pneumoniae) is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.	Product	Price	Quantity
SPT4001	S. pneumoniae Topo IV100U	£136
SPT4002	S. pneumoniae Topo IV500U	£498

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These contain S. pneumoniae topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.   

Technical Documents

Cat No.	Product	Price	Quantity
SPR4001	S.pneumoniae Topo IV RelaxationAssay Kit 1100 U of S.pneumoniae Topo IV and 50 µg of Supercoiled DNA	£220
SPR4002	S.pneumoniae Topo IV RelaxationAssayKit 2500 U of S. pneumoniae Topo IV and 250 µg of Supercoiled DNA	£817

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These contain S. pneumoniae topo IV and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.   

Technical Documents

Cat No.	Product	Price	Quantity
SPD4001	S.pneumoniae Topo IV Decatenation AssayKit 1100 U of S.pneumoniae Topo IV and 20 µg of kDNA	£220
SPD4002	S.pneumoniae Topo IV Decatenation Assay Kit 2500 U of S.pneumoniae Topo IV and 100 µg of kDNA	£817

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